Figure 2

Electrostatic surface of catB in distinct protonation states reveals two possible heparin binding sites The electrostatic surface of catB using protonation states corresponding to acidic and alkaline pH conditions. Blue, red, and white indicate positively charged regions, negative areas and neutral regions, respectively, with the scale indicating ± k_bT/e_c, where k_b is the Boltzmann constant, T is the temperature and e_c is the charge of one electron. The heparin binding sites correspond to the basic regions localized in each domain.