Figure 5

Alignment extension with PfFSmat60 matrix for P. berghei probable bi-functional enzyme of shikimate pathway. The sequences compared here are the P. bergheii probable bi-functional enzyme having EPSP and SK activities, PBANKA_030400 and well characterized yeast multifunctional protein, Aro1p (gi:6320332). (a) The alignment with BLOSUM50 showing the aligned motif regions for only EPSP synthase I motif (gray shading). (b) The alignments extended by PfFSmat60 for both the EPSP synthase I and shikimate kinase motifs are represented as (i) and (ii) respectively. The water program (EMBOSS package, version 6.3.1) was used for alignment.