Figure 5

Correlation between qPCR and coverage. The average coverage across a fragment amplified by qPCR is compared to the copy number, measured by the ΔCt of the qPCR reaction relative to the average Ct value of six single copy B. malayi genes. The error bars for the average coverage are one standard deviation. The error bars for the copy number are derived from one standard deviation of the ΔCt, making them asymmetric since copy number is exponentially related to ΔCt.