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Table 1 Quality control measures as a function of starting material.

From: Reproducibility of oligonucleotide arrays using small samples

Ā 

Amount of starting RNA

Ā 

10 Ī¼g

2 Ī¼g

0.5 Ī¼g

0.1 Ī¼g

% Present

38.6%

37.8%

30.2%

24.5%

Scaling Factor

14.1

14.3

33.4

89.0

3'/5' GAPDH

1.7

1.6

6.1

5.5

3'/5' Ī²-actin

1.5

1.5

9.2

8.2

  1. Data are averages of all arrays in each RNA sample size group. 3'/5' signal ratios are given for GAPDH and Ī²-actin.
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BMC Genomics

ISSN: 1471-2164

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