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Table 3 Variability in the detection of probe sets.

From: Reproducibility of oligonucleotide arrays using small samples

Ā 

Changes in Detection1

Ā 

<3002

300–6002

>6002

Ā 

P10 to A3

A10 to P4

P10 to A3

A10 to P4

P10 to A3

A10 to P4

2.0 μg

104 (58%)

131 (74%)

51 (28%)

35 (20%)

25 (14%)

1 2 (7%)

0.5 μg

241 (28%)

128 (74%)

245 (29%)

32 (18%)

365 (43%)

1 4 (8%)

0.1 μg

346 (28%)

52 (68%)

355 (29%)

15 (19%)

523 (43%)

10 (13%)

  1. 1Changes in Dectection are the average number of Present to Absent and Absent to Present changes per chip when comparing the 10 μg chip to the reduced RNA sample chip from the same animal. 2Number of changes found among those probe sets that had a signal less than 300, 300 to 600 or greater than 600 (as noted) in the 10 μg chip. 3Present in 10 μg chip, absent in reduced RNA sample chip from the same animal. 4Absent in 10 μg chip, present in reduced RNA sample chip from the same animal.