Fig. 1

Cappable-seq pipeline for TSS identification. a Schema of Cappable-seq protocol and the associated control library. b Replicate analysis. The correlation coefficient between replicate 1 and replicate 2 RRS is 0.983. c Enrichment score as a function of the mean of relative read score for the 36078 putative TSSs found in E. coli grown on minimal media. In blue are TSS that are enriched in Cappable-seq library. Grey are positions that are depleted in Cappable-seq. The removal of depleted positions eliminates 1354 spurious TSS primarily located in ribosomal loci