Fig. 1

Scheme for generation of MSB and NSB. Rice calli were transformed by Agrobacterium. The transgenic line 51A was self-pollinated for five generations to fix the transgene, and the seeds of the sixth generation produced by T5 plants were defined as the master seed bank (MSB). T6 plants were grown from part of the MSB seeds and self-pollinated to produce the next seed bank (NSB; T7 seeds) in a fully closed-type plant production facility built in IMSUT