Table 1 Ranked list of the most significant genes / COGs associated with greater biofilm growth at 22^∘C than 37^∘C using Scoary and Pyseer

COG / Gene

Function

Presence

OR

Ben p

Example (isolate_gene position)

1

group_8321

Methyltransferase

68

6.389

0.018

WH-SGI-V-07050_00375

2

group_15072

Hypothetical

80

5.044

0.018

WH-SGI-V-07050_00461

3

group_1519

SAM-dependent methyltransferase

79

4.932

0.018

WH-SGI-V-07050_00374

4

group_15041

Integrating conjugative element protein

75

5.251

0.018

WH-SGI-V-07050_00387

5

group_11043 (arsC_1)

Arsenate reductase (glutaredoxin)

78

4.823

0.018

WH-SGI-V-07050_00379

6

acr3

ACR3 family arsenite efflux transporter

78

4.823

0.018

WH-SGI-V-07050_00380

7

arsC_2

Arsenate reductase

78

4.823

0.018

WH-SGI-V-07050_00381

8

group_11046

ArsR family transcriptional regulator

78

4.823

0.018

WH-SGI-V-07050_00382

9

group_2314

Hypothetical

71

4.823

0.018

WH-SGI-V-07050_00373

10

arsH_1

Arsenical resistance protein

78

4.823

0.018

WH-SGI-V-07050_00378

11

ydfG

NADP-dependent 3-hydroxy acid dehydrogenase

73

5.015

0.018

WH-SGI-V-07050_00440

12

group_15062

TetR/AcrR family transcriptional regulator

73

5.015

0.018

WH-SGI-V-07050_00441

13

group_11053

Integrating conjugative element relaxase, PFGI-1 class

77

4.715

0.018

WH-SGI-V-07050_00417

14

qorB

Quinone oxidoreductase

77

4.715

0.018

WH-SGI-V-07050_00419

15

group_832

Hypothetical

77

4.715

0.018

WH-SGI-V-07050_00384

16

group_11042

Hypothetical

63

5.658

0.018

WH-SGI-V-07050_00376

17

group_15038

Integrating conjugative element protein pill, pfgi-1

76

4.608

0.018

WH-SGI-V-07050_00383

18

group_15039

TIGR03759 family integrating conjugative element protein

76

4.608

0.018

WH-SGI-V-07050_00385

19

group_15040

Transglycosylase SLT domain-containing protein

76

4.608

0.018

WH-SGI-V-07050_00386

20

group_11058

MgtC/SapB family protein

84

4.203

0.018

WH-SGI-V-07050_00439

COG / Gene

Function

Presence

Beta

lrt p

Example

1

group_8321

Methyltransferase

68

1.9200

0.0001

WH-SGI-V-07050_00375

2

group_15072

Hypothetical

80

1.6800

0.0001

WH-SGI-V-07050_00461

3

group_15041

Integrating conjugative element protein

75

1.6500

0.0002

WH-SGI-V-07050_00387

4

group_11114

Hypothetical

140

1.5000

0.0002

WH-SGI-V-07050_02801

5

group_1519

SAM-dependent methyltransferase

79

1.5700

0.0003

WH-SGI-V-07050_00374

6

group_3009

4-alpha-glucanotransferase

72

1.3700

0.0003

WH-SGI-V-07051_05729

7

clsA

Major cardiolipin synthase ClsA

42

1.6900

0.0003

WH-SGI-V-07066_05171

8

ydfG

NADP-dependent 3-hydroxy acid dehydrogenase

73

1.6200

0.0003

WH-SGI-V-07050_00440

9

group_15062

TetR/AcrR family transcriptional regulator

73

1.6200

0.0003

WH-SGI-V-07050_00441

10

group_11043 (arsC_1)

Arsenate reductase (glutaredoxin)

78

1.5500

0.0003

WH-SGI-V-07050_00379

11

acr3

ACR3 family arsenite efflux transporter

78

1.5500

0.0003

WH-SGI-V-07050_00380

12

arsC_2

Arsenate reductase

78

1.5500

0.0003

WH-SGI-V-07050_00381

13

group_11046

ArsR family transcriptional regulator

78

1.5500

0.0003

WH-SGI-V-07050_00382

14

arsH_1

Arsenical resistance protein

78

1.5500

0.0003

WH-SGI-V-07050_00378

15

fliC

Flagellin

109

1.2400

0.0004

WH-SGI-V-07064_05796

16

group_5435

Alpha/beta fold hydrolase

101

-1.1300

0.0004

WH-SGI-V-07049_05847

17

group_5843

Hypothetical

35

1.8200

0.0004

WH-SGI-V-07066_05167

18

group_2314

Hypothetical

71

1.5400

0.0004

WH-SGI-V-07050_00373

19

group_832

Hypothetical

77

1.5100

0.0004

WH-SGI-V-07050_00384

20

group_11053

Integrating conjugative element relaxase, PFGI-1 class

77

1.5000

0.0005

WH-SGI-V-07050_00417