Table 1 Study design for iM line snails used for RNA-Seq. All snails were juveniles (5–8 mm shell diameter) at the start of this study. All parasite infections were administered on the same day. Three snails were sequenced for each of the indicated combinations of parasite species vs. days-post infection. Snails infected for 2 or 8dpi were juveniles. By 40dpi, parasite-infected snails were of adult size (> 10 mm shell diameter). Additionally, 3 juvenile and 3 adult control snails, treated identically to parasite-infected snails except they were not exposed to parasites, were sampled at the indicated times. In total, 33 snails were subjected individually to RNA-Seq

From: Different metazoan parasites, different transcriptomic responses, with new insights on parasitic castration by digenetic trematodes in the schistosome vector snail Biomphalaria glabrata