Fig. 2

The α-globin genes (OMIM: NG_000006.1) with primers map. a) Map of α-globin genes (HbA2 and HbA1), the -α^3.7 deleted allele and the -α^3.7 primer set. For normal allele, primers P1 and P2 give rise to 303 bp and 311 bp fragments and primers P3 and P4 give rise to 252 bp fragment. For -α^3.7 deleted allele, primers P1 and P2 give rise to only 311 bp fragment and primers P3 and P4 do not have any template to amplify (Tables 2 and 1). Map of α-globin genes (HBM, HBAP1, HBA2, HBA1 and HBQ), the -α^SEA deleted allele, and the -α^SEA primer set [29]. For normal allele, primers W1 and W2 give rise to 288 bp and primers W3 and W4 give rise to 130 bp. For -α^SEA deleted allele, primers W1 and W4 give rise to 217 bp fragment (Tables 2 and 1)