Fig. 3

The results of the novel Hb H disease^{− SEA/−3.7kb} genotyping protocol of each α-thalassemia genotype. A shows the results of normal α-globin (αα/αα) genotype with positive normal fragments i.e. blue 303 bp HBA2, blue 311 bp HBA1, blue 252 bp internal control by the -α^3.7 primer set and normal blue 288 bp 5’ breakpoint and green 130 bp 3’ breakpoint by the -α^SEA primer set from the normal αα allele. B shows results of the compound heterozygous Hb H disease^{− SEA/−3.7kb} with positive only blue 303 bp HBA2 fragment from -α^3.7 allele and normal blue 288 bp 5’ -SEA breakpoint and green 130 bp 3’ -SEA breakpoint and blue/green 217 bp mutant fragment. C shows the results of heterozygous − 3.7 kb (-α^3.7/αα) genotype with positive normal fragments i.e. blue 303 bp HBA2, blue 311 bp HBA1, blue 252 bp internal control and normal blue 288 bp 5’ -α^SEA breakpoint and green 130 bp 3’ -SEA breakpoint. D shows results of the homozygous − 3.7 kb (-α^3.7/-α^3.7) genotype with positive only blue 311 bp HBA1 fragments and normal blue 288 bp 5’ -SEA breakpoint and green 130 bp 3’ -SEA breakpoint. E shows results of the heterozygous -SEA deletion (--^SEA/αα) genotype with positive normal fragments i.e. blue 303 bp HBA2, blue 311 bp HBA1, blue 252 bp internal control and normal blue 288 bp 5’ -SEA breakpoint and green 130 bp 3’ -SEA breakpoint and blue/green 217 bp mutant fragment from the --^SEA allele. F shows results of the homozygous -SEA deletion (--^SEA/--^SEA) genotype with positive only blue/green 217 bp mutant fragment from the --^SEA allele