Development of pre-implantation genetic testing protocol for monogenic disorders (PGT-M) of Hb H disease

BMC Genomics

Table 1 The primers details for -α^3.7 and -α^SEA protocols for PGT-M of Hb H disease^{− SEA/−3.7kb}

Primer sets	Locations	Primers	Sequences	Labeling Dyes	Products (bp)	References
-α^3.7 primers	HBA2 & HBA1	P1	5’-GGGTTGCGGGAGGTGTAG-3’	6’FAM	303 (HBA2), 311 (HBA1)	NG_000006.1
	HBA2 & HBA1	P2	5’-CCCACTCAGACTTTATTCAAAGACC-3’	-	303 (HBA2), 311 (HBA1)
	Internal Control HBA	P3	5’-TGACCTGATGCACTCCTCAA-3’	6’FAM	252
	Internal Control HBA	P4	5’-TGTGGTTGGAGAATGGAGGT-3’	-	252
-α^SEA primers [29]	Normal 5’ Breakpoint	W1	5’-GAAGGAGGGGAGAAGCTGAG-3’	6’FAM	288	NG_000006
	Normal 5’ Breakpoint	W2	5’-TGTGGAAAAGTTCCCTGAGC-3’	-	288
	Normal 3’ Breakpoint	W3	5’-TGCACACCTATGTCCCAGTT-3’	-	130
	Normal 3’ Breakpoint	W4	5’-TTGAGACGATGCTTGCTTTG-3’	VIC	130
	Deleted Mutant	W1	5’-GAAGGAGGGGAGAAGCTGAG-3’	6’FAM	217
	Deleted Mutant	W4	5’-TTGAGACGATGCTTGCTTTG-3’	VIC	217

ISSN: 1471-2164