BMC Genomics

Table 2 Interpretation table of -α^3.7 and -α^SEA protocols for Hb H disease^{− SEA/−3.7kb} genotyping

From: Development of pre-implantation genetic testing protocol for monogenic disorders (PGT-M) of Hb H disease

Primer Sets	Fragments	Genotypes
Primer Sets	Fragments	αα/αα	-α^3.7/αα	-α^3.7/-α^3.7	--^SEA/αα	--^SEA/--^SEA (Hb Bart’s)	--^SEA/-α^3.7 (Hb H)
-α^3.7 primers	HBA2/HBA1: P1/P2 303/311 bp	303/311	303/311	- / 311	303/311	-	- /311
-α^3.7 primers	Internal HBA: P3/P4 252 bp	+	+	-	+	-	-
-α^SEA primers [29]	5’ Breakpoint: W1/W2 288 bp	+	+	+	+	-	+
	Deleted Mutant: W1/W4 217 bp	-	-	-	+	+	+
	3’ Breakpoint: W3/W4 130 bp	+	+	+	+	-	+

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ISSN: 1471-2164

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