Fig. 8

Differential analysis of the mRNA expression levels between the control group and the strain cB07 inoculation group. (a) Principal component analysis of RNA-seq data. The points represent biological replicates. (b) A histogram of differential gene expression among different groups. (c, d) Volcano plots showing the relative abundance of transcripts. The transcripts were considered differentially expressed between groups at fold change ≥ 2 and with a FDR < 0.05. The black points represent genes for which no statistically significant differences were observed. The blue dots represent significantly downregulated genes and the orange dots represent significantly upregulated genes. CM, Metamorphosis, control (CK) group; BM, Metamorphosis, bacteria inoculation (Bac) group; CP, Settlement, CK group; BP, Settlement, Bac group