Fig. 2

Validation of transcript expression using RT-qPCR analysis. selected transcripts from differentially expressed genes and hormone signalling pathways (A) Non-symbiotic hemoglobin 2 (MSTRG.298.1) (B) Putative norcoclaurine 6-O-methyltransferase (MSTRG.1147.1) (C) Corytuberine synthase (MSTRG.15304.2) (D) Fe2OG dioxygenase domain-containing protein (MSTRG.13487.1) (E) WRKy70 (MSTRG.8862.1) (F) MYC2 (MSTRG.4905.1) (G) TGA4 (MSTRG.4306.1) (H) MPK4 (MSTRG.830.2) were quantified in three samples using RT-qPCR. The grey-scale bars represent relative enzyme coding transcript expression in control, 6 hpi and 12 hpi by RT-qPCR analysis (left y-axis). The data represents mean values of three biological replicates for each sample. Black line represents TPM values of the transcripts by RNAseq (right y-axis)