Table 2 Comparison of differentially-expressed genes involved in the biosynthesis of Dufour’s gland ester signals identified in a previous RNA-seq study [14] with protein expression in the current study. Pairwise comparisons were conducted between queens, gynes, queenless workers (QLW), and queenright workers (QRW). For each pairwise comparison, we provide the log2 fold change (logFC) and FDR adjusted p-value (adjP) in both RNAseq and proteomic studies. Cells shaded in teal show comparisons with concordance between proteomics and RNA-seq studies (i.e., the transcripts and proteins were significantly up or down regulated in the same direction). Orange cells show comparisons where the protein was differentially expressed but the transcript was not. Unshaded cells contain values which were either only differentially expressed in the RNAseq study or in neither study
From: Post-transcriptional regulation of Dufour’s gland reproductive signals in bumble bees
Queen vs. Gyne | Queen vs. QLW | Queen vs. QRW | Gyne vs. QRW | Gyne vs. QLW | QLW vs. QRW | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Proteomics | RNA-seq | Proteomics | RNA-seq | Proteomics | RNA-seq | Proteomics | RNA-seq | Proteomics | RNA-seq | Proteomics | RNA-seq | ||||||||||||||
Accession | Description | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP | logFC | adjP |
A0A6P6F860 | Fatty acyl-CoA reductase | 2.98 | 0.002 | 9.83 | 0.000 | 2.51 | 0.002 | 7.43 | 0.000 | 2.40 | 0.051 | 8.67 | 0.000 | -0.28 | 0.970 | -1.16 | 0.659 | 0.00 | 0.966 | -2.40 | 0.264 | -0.26 | 0.434 | 1.23 | 0.958 |
A0A6P8M112 | Fatty acyl-CoA reductase | 0.04 | 0.999 | 3.06 | 0.038 | -3.09 | 0.622 | -3.08 | 0.181 | -5.99 | 0.000 | -3.57 | 0.111 | -6.89 | 0.000 | -6.63 | 0.003 | -3.39 | 0.051 | -6.15 | 0.003 | -2.15 | 0.000 | -0.49 | 0.991 |
A0A6P3DW33 | Very-long-chain enoyl-CoA reductase | -2.16 | 0.633 | 0.28 | 0.565 | -4.07 | 0.025 | -0.60 | 0.292 | -4.75 | 0.000 | -0.77 | 0.145 | -3.15 | 0.016 | -1.05 | 0.037 | -2.89 | 0.081 | -0.88 | 0.072 | -0.04 | 0.626 | -0.16 | 0.981 |
A0A6P3V0 × 0 | acyl-CoA Delta(11) desaturase isoform X2 | -3.63 | 0.032 | -1.21 | 0.075 | -0.91 | 0.989 | 1.20 | 0.088 | -1.51 | 0.565 | 0.70 | 0.319 | 1.49 | 0.925 | 1.91 | 0.006 | 2.72 | 0.014 | 2.41 | 0.001 | -0.80 | 0.131 | -0.50 | 0.958 |
A0A6P3V078 | 3-hydroxy-3-methylglutaryl coenzyme A reductase | -0.74 | 0.999 | -0.06 | 0.969 | 1.50 | 0.191 | 3.49 | 0.001 | 2.05 | 0.196 | 3.13 | 0.003 | 3.32 | 0.097 | 3.19 | 0.002 | 2.43 | 0.027 | 3.54 | 0.001 | 0.67 | 1.000 | -0.35 | 0.975 |
A0A6P3UUI7 | Fatty acyl-CoA reductase | -2.45 | 0.506 | -4.69 | 0.130 | 1.20 | 0.134 | 3.50 | 0.119 | -0.62 | 1.000 | 1.97 | 0.412 | 2.39 | 0.466 | 6.66 | 0.027 | 4.92 | 0.000 | 8.19 | 0.006 | -2.32 | 0.000 | -1.53 | 0.958 |
A0A6P3UTI4 | Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase | -1.27 | 0.982 | -2.42 | 0.000 | -2.83 | 0.723 | -2.08 | 0.000 | -3.62 | 0.002 | -1.72 | 0.000 | -1.86 | 0.201 | 0.70 | 0.126 | -1.26 | 0.966 | 0.34 | 0.495 | -0.60 | 0.253 | 0.36 | 0.958 |
A0A6P8LCZ2 | Hydroxymethylglutaryl-CoA synthase | -3.13 | 0.136 | -2.59 | 0.002 | -0.10 | 0.865 | 0.55 | 0.527 | 0.29 | 1.000 | -0.94 | 0.241 | 3.59 | 0.062 | 1.65 | 0.060 | 3.04 | 0.005 | 3.14 | 0.000 | 0.42 | 0.940 | -1.49 | 0.925 |
A0A6P8L4 × 3 | Fatty acyl-CoA reductase | -0.96 | 0.999 | 2.92 | 0.091 | -4.76 | 0.009 | -2.99 | 0.045 | -7.21 | 0.000 | -3.40 | 0.021 | -5.88 | 0.000 | -6.32 | 0.002 | -4.79 | 0.000 | -5.92 | 0.003 | -2.46 | 0.000 | -0.40 | 0.986 |