Fig. 4

Anatomical structure development-associated gene expression patterns. (A, B) Expression patterns throughout development of and GOslims associated with clusters 8 (n = 176) and 12 (n = 51), respectively. GOslim results from cluster 8 reveal that muscle structure development occurs from 132 hours after egg laying (hAEL) onwards and cluster 12 reveals a peak in respiratory system development at 48 hAEL and from 132 hAEL onwards. Each timepoint shows mean values of biological replicates (n = 1350 eggs). Total mean values are depicted in black. (C) Developmental expression levels of eye related genes. gl, glass; chp, chaoptin; LWS2/4, Long wavelength-sensitive cone opsin 2/4; SWSB3, Short wavelength-sensitive B opsin 3; Rh6, Rhodopsin 6. Expression data presented in log2-centered TMM (+ 1) values.