Fig. 1

Reduced reproductive performance in old mice. A) Comparison of litter size in young and old mice. B) Decreased proliferation of granulosa cells in older mice. C–E) Cell cycle analysis in young and old mice. F–H) The mRNA (F) and protein (G-H) expression levels of CDK1, HAS2, TNFAIP6 and PTX3 in GCs. (n = 3). *P < 0.05, **P < 0.01