Table 1 Primer sequence for RT-qPCR

Has2

(mouse)

F: 5′- TGCTGGTTCAGCCATCTCAG − 3′

R: 5′- GACGACAGGCACCTTACCAA-3′

Tnfaip6

(mouse)

F: 5′- TTCCATGTCTGTGCTGCTGGATGG-3′

R: 5′- AGCCTGGATCATGTTCAAGGTCAAA-3′

Ptx3

(mouse)

F: 5′- GTGGGTGGAAAGGAGAACAA − 3′

R: 5′- GGCCAATCTGTAGGAGTCCA-3′

CDK1

(mouse)

F: 5′- AGAAGGTACTTACGGTGTGGT − 3′

R: 5′- GAGAGATTTCCCGAATTGCAGT − 3′

DNMT1

(mouse)

F: 5′- GAGCCCTATCGCATCGGT-3′

R: 5′- GGCCCCCTTGTGAATAATC-3′

GAPDH

(mouse)

F: 5′- AGGTCGGTGTGAACGGATTTG-3′

R: 5′- TGTAGACCATGTAGTTGAGGTCA-3′

TGFb2

(pig)

F: 5′- CGATGATGATGTTGATGATGG − 3′

R: 5′- GCAAGGCTTTCTTGTATTTTCTTG-3′

E2F1

(pig)

F: 5′- GCCAAGAAGTCCAAGAACC-3′

R: 5′- GCATCCTCGGAAAGCAG-3′

BUB1

(pig)

F: 5′- TCAAAGGGCAGGTTGCAGAA-3′

R: 5′- TGACTTCACTCGGAACACCG-3′

CDKN1A

(pig)

F: 5′- CGAGAGCGATGGAACTTCGACTTC-3′

R: 5′- TCCACATGGTCCTCCTGAGACG-3′

GAPDH

(pig)

F: 5′- AGGTCGGTGTGAACGGATTTG-3′

R: 5′- TGTAGACCATGTAGTTGAGGTCA-3′