Table 1 Primers used for mRNA-seq verification. Tm: primer-specific annealing temperature
Category | Description | Unigene ID | Primer sequences (5’→3’) | Tm(℃) |
|---|---|---|---|---|
Reference gene | 18Â S rRNA | Â | F: TCTTGATTCGGTGGGTGGTG R: CCCGGACATCTAAGGGCATC | 60 |
mRNA | Cyclin-dependent kinase 20 | Cluster-26337.54349 | F: GACGGAGGCCCAGGTCAAATCGTACA R: AGCAGCCCACAGGTCGACTCCTTCAT | 61 |
| Â | Cyclin-dependent kinase 13-like | Cluster-26337.77830 | F: CAACAACAGGGGTCAGATCAAGCTGGG R: GGTGCTGTCTCTTGGGCTTGAAGGCA | 61 |
| Â | Cyclin-dependent kinase 6-like | Cluster-26337.23585 | F: TACAAGGCTCGGGACCTGCGTAACCA R: GAGGCCAGGTCATGCTCGATGTACTC | 61 |
| Â | Cyclin-dependent kinase 11B-like isoform X2 | Cluster-26337.71332 | F: CCGTATAAGAGAACGCCCCCGTAGAG R: GTGTTCTTCTCATGGTGCACAGGCG | 61 |
| Â | Cyclin-dependent kinase 2-like | Cluster-26337.55501 | F: CCAGAACCTCCTCATCGACTCCAAGAT R: GGGGTGCCCAAAGTTCTGAAGATTCGG | 61 |
| Â | Cyclin-dependent kinase 8-like isoform X8 | Cluster-26337.26275 | F: CGGGTTTTCCTGTCCCATACAGACCG R: GAGGCTTGAGTGGGGAGTTGAAGAGAC | 61 |
| Â | Cyclin-dependent kinase 13-like | Cluster-26337.85551 | F: CGGCCTGCACTACTGCCACCAGAAAA R: GCAAACTCCTCCTTGGCCCGGAAGAT | 61 |
| Â | Cyclin-dependent kinase 16-like isoform X1 | Cluster-26337.84189 | F: ACATCATGGACCAGCAGTCCCCCAAAC R: TGTTGGGTGACGGCAGAGGAAGAGGA | 61 |
| Â | Cyclin-dependent kinase 1-like | Cluster-26337.70194 | F: GGCCGCCACAGGAGAACAAATCAGCTT R: GTTCTGAGGCTTGAGATCCCGATGCAG | 61 |
| Â | Cyclin-dependent kinase-like 5 isoform X1 | Cluster-26337.90933 | F: GGACCACAATGAGAGAGCTGACCATGC R: CCCAGTTGTGCCGTTGCTGATGTTTCG | 61 |
| Â | Cyclin-dependent kinase 1 | Cluster-26337.69678 | F: TTGACTCCTCCAGAAGACGACA R: TGGCTTGAGATCACGGTGC | 61 |
| Â | Cyclin-dependent kinase 8-like isoform X4 | Cluster-26337.5567 | F: TATCATCAAGTTCCACCGTTCA R: AGAGGCGAGCAAAACCCA | 61 |