Fig. 10

Overview of the loss and retention of key genes involved in the glycolytic pathway genes across different microsporidia species. Retained genes are highlighted in light blue in the heatmap, while those that have been lost are highlighted in gray. Microsporidia sp. MB has retained most of the genes involved in glycolysis except for Pyruvate kinase, similar to V. corneae and N. granulosis. Comparatively, E. aedis, E. cuniculi, and E. hepatopenaei appear to have lost all these genes, suggesting a total reliance on the host for energy production. The maximum likelihood phylogenomic tree on the left was generated using IQ-TREE v1.6.12 [85] based on the 196 common BUSCO single-copy orthologous genes found in all species. Percent bootstrap support values are shown on each node and the branch length scale is bare at the bottom of the tree