Fig. 7

Distribution of the SNPs and their frequencies alongside the intron-4–exon-5 boundary of the doublesex gene; the rectangle on bottom shows the intron-4-exon-5 boundary of the doublesex gene (dark red: exon-5 untranslated sequence, gray: exon-5 translated sequence and simple gray line: intron-4); the y-axis shows the allele frequencies of the SNPs and the x-axis shows the positions of the SNPs in the 2R chromosome; the two vertical dashed lines are delimiting the Ag(QFS)1 or CRISPR/Cas9 target sequence; The DNA sequences at the bottom shows the SNPs positions alongside the reference genome in the gRNA target sequence within different populations. GD: « Gene Drive »; Amplicon seq. data: Amplicon sequencing data from laboratory (Lab) and wild-caught mosquito samples from 8 villages in Burkina Faso; Ag1000G data: Whole genome sequencing data of Anopheles gambiae 1000 genomes Consortium from wild caught An. gambiae s.l. mosquitoes collected in 3 villages from Burkina Faso