Fig. 1

EGF modifies the IFN-g-regulated transcriptome and globally attenuates IFN-g-induced gene expression in human keratinocytes. a Heatmap showing differentially expressed genes (FDR < 0.01) induced by IFN-g alone, EGF alone, or IFN-g plus EGF (excluding genes that were not differentially expressed in any treatment group relative to control). FDR distributions and volcano plots (with dashed line corresponding to FDR cut-off of 0.01) showing log2 fold change in gene expression, relative to control, in keratinocytes following treatment with (b) IFN-g alone, and (c) IFN-g plus EGF. d Venn-diagram showing differentially expressed genes (FDR < 0.01) induced by IFN-g and/or EGF. e Fold change of the top 10 genes induced after treatment with IFN-g alone; asterisks above CXCL10 and GBP5 correspond to statistically significant interaction FDR values (*FDR < 0.05; ** FDR < 0.01) and indicate that EGF modified (attenuated) the differential gene expression induced by IFN-g (detailed information in Supplementary Table 3)