Fig. 3

Characteristics of conserved TFBSs. (A) Number of P. vulgaris genes that have orthologs, similar promoters, and conserved TFBSs with each of the compared species. (B) Number of shared conserved TFBSs between all studied species. C) Number of conserved TFBSs by TF families. TFBSs present in P.vulgaris and at least one other species are included. Each bar represents the number of TFBS belonging to a specific family. The black portion of the bar indicates the number of conserved sites that do not overlap with annotated upstream gene, while the portion area shows the number of sites located within the body of an upstream gene. (D) Distribution in the number of conserved TFBSs across TSS upstream regions by their conservation level. A higher conservation level indicates that a TFBS is present in P. vulgaris promoter and on promoter regions of multiple studied species. For example, conservation level of 1 means that the TFBS is conserved between P. vulgaris and one other species, while conservation level of 3 indicates that the site is present in all studied species. (E) Distribution of conserved TFBSs across promoters is assessed based on their overlap with annotated features. The gray line represents the number of conserved sites located within the body of the gene, excluding the flanking genes