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Fig. 2 | BMC Genomics

Fig. 2

From: Histone H3 K4 trimethylation occurs mainly at the origins of polycistronic transcription in the genome of Leishmania infantum promastigotes and intracellular amastigotes

Fig. 2

Evaluation of the ability of the anti-H3K4me3 antibody used in the study to recognize its target in L. infantum. (A) Total lysates from L. infantum promastigotes (P), amastigotes (A) and human acute promyelocytic leukemia NB4 cell line were separated by SDS-PAGE and stained with Coomassie Brilliant Blue to observe histones separation pattern. Putative Leishmania histone H3 (~ 15 kDa) and human histone H3 (~ 16 kDa) are indicated by arrows. (B) Western blot analysis of whole-cell lysates of L. infantum promastigotes (P), amastigotes (A) and NB4 cell line performed with the anti-H3K4me3 antibody used in the study (upper line) and an anti-histone H3 antibody (lower line)

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BMC Genomics

ISSN: 1471-2164

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