Fig. 3

A Relative gene expression of SmVALs across the S. mansoni life cycle. The three datasets of expression counts for each stage were obtained as described in the Methods section. The heatmap shows the means of GeTMM-normalized counts, allowing for intra- and intersample comparisons. Newly identified SmVALs within this study are in bold. To add phylogenetic context to expression patterns across the life cycle, a proportionally transformed tree from Fig. 1 was added. B Gene expression of the SmVAL family compared between immature and mature S. mansoni eggs from the intestinal and liver tissues of experimentally infected mice. The heatmap shows the mean gene expression levels normalized to reads per million (RPM), which highlights the proportional contributions of each subgroup to the overall gene expression profile in a given sample. The expression data were obtained from Supplementary Table S2 of Peterková et al. 2024 [21]