Fig. 5

Immunolocalization SmVAL9 and SmVAL29 in the sections of S. mansoni infected mouse liver and intestine. Confocal close-ups illustrate mature eggs with developed miracidia. The red signal represents anti-SmVAL serum-specific sites, the green signal indicates eggshell autofluorescence, and the blue signal indicates the DAPI-labelled cell nuclei. Both SmVAL proteins are localized in the miracidia penetration glands and the surface of the developed larvae. There was no specific protein signal in the control serum obtained from an non-immunized mouse