Fig. 6

SAVEXIS plates for SmVAL9 and SmVAL29 preys tested against a library of 755 human receptors. A library of 755 human receptors was arrayed across two 384-well plates (plates A and B). Controls are located in the bottom right corner of each plate. OX68 (well P24 on plates A and B) and anti-His (well O24 on plate B) are two biotinylated antibodies used as baits and target the Cd4 tag and 6 × His tag of the recombinant proteins, respectively. They act as positive controls by capturing prey through the relevant tags. CD4 (well P23 on plates A and B) corresponds to the biotinylated rat Cd4 (d3 + 4) tag used as bait. It acts as a negative control, and any signal observed in this well is considered background noise from the prey. CD200R (well O24 in plate A and N24 in plate B) is a positive control interaction between rat Cd200, which is used as bait, and rat Cd200R, which is used as prey. A mutated human LAIR1 ectodomain (well M23 in plate B) was included as bait in the SAVEXIS assay and proved to be very promiscuous, binding to > 75% of all preys tested in the assay