Fig. 3
From: Cas9-expressing cattle using the PiggyBac transposon all-in-one system
Genetic analyses of F1 PB-Cas9-GFP-sgPRNP calves. AÂ GFP expression was confirmed in primary cells from the ear tissue of calves #G3-1 and #G3-3. BÂ PCR revealed transgenic integration in calves #G3-1, #G3-3, and #G7-1. CÂ Western blotting to detect the Cas9 protein in calves #G3-1 and #G3-3. DÂ T7E1 assay revealing that the PRNP mutation was exclusively identified in calf #G7-1