Altered structural and transporter-related gene expression patterns in the placenta play a role in fetal demise during Porcine reproductive and respiratory syndrome virus infection

Table 3 Gene groupings, gene targets tested within each group, number of differentially expressed genes (DEG), and gene grouping enrichment

Gene group	Gene targets within group	Total DEG	Number of genes tested	Enrichment (%)
Actin cytoskeleton signaling	*ACTA4, ACTG1, ARHGEF6, ARHGEF7, ARPC1A, ARPC2, CDC42, F2RL1, IQGAP, LIMK2, RAC2, ROCK1, ROCK2*	5	13	38%
Arachidonic acid	CBR1, CBR2, *CBR3, PTGES, PTGES2, PTGES3, PTGIS, PTGS1, PTGS2, TBXAS1, PLA2G4A*	6	11	55%
Integrin signaling	*FN1, GRB2, ITGA2, ITGAV, ITGB1, ITGB2, ITGB3, ITGB4, ITGB5, ITGB6, ITGB7, ITGB8, SPP1*	10	13	77%
Intracellular junctions	CDH1, *CDH11, CDH3, CDH5, DSC1, DSC2, DSC3, DSG1, DSG2, DSG3, TJP1, TJP2, TJP3*	8	13	62%
Transporters	*SLC1A3, SLC1A5, SLC2A1, SLC2A2, SLC2A3, SLC2A5, SLC2A10, SLC2A12, SLC7A1, SLC7A4, SLC16A1, SLC16A3, SLC16A5, SLC16A10, SLC27A4, SLC27A6*	14	16	88%
VEGF signaling	*ARRB1, ARRB2, BCL2L14, FLT1, HIF1A, HPSE, KDR, MAPK1, MAPK3, NOS1, NOS2, NOS3, PGF, PLCG1, PLCG2, PTGR1, VEGFA, VEGFB, VEGFC, VEGFD*	11	20	55%
House Keeping	HMBS, IPO8, MAU2, RPL32	4	0	N/A

Each group of fetuses was contrasted with the control group. Group control (CTRL) was mock infected, viable and low virus fetus (VIA_LVF), viable and high virus fetus (VIA_HVF), meconium mild and low virus fetus (MECm_LVF), meconium mild and high virus fetus (MECm_HVF), and meconium severe and high virus fetus (MECs_HVF). Analysis for DEG was completed in Limma and those reported had a P ≤ 0.05. Bolded genes indicated they were identified as DEG in ≥ 1 contrast compared to the CTRL group. Enrichment % was calculated as the number of DEG divided by the number of genes tested multiplied by 100

ISSN: 1471-2164