Fig. 4

Differential expression of C2H2 Zinc Finger family transcription factors in AML cell lines. (A) Bar charts indicating the percentage of genes specifically up- or down- regulated in each cell line which are members of the C2H2 ZNF family. The dashed line indicates the overall percentage of this family across the genome (B) Bar charts indicating the number of total and differentially expressed C2H2 ZNF genes on each chromosome. Differentially expressed ZNFs are those that showed differential expression (in the same direction) between one cell line and all other three cell lines in pairwise comparisons as shown in Fig. 2. (C) Heatmap representation of the expression of ZNF genes within cluster 2 (left) and cluster 5 (right) on chromosome 19 (scaled within rows). Hierarchical clustering was applied only to columns (samples) and ZNF genes are shown according to their physical position within the gene cluster. Brackets indicate sub-clusters of physically-proximal genes showing co-ordinated expression profiles across the cell lines as described in the text. (D-F) Bar charts showing expression (TPM) of the indicated ZNF genes from different subclusters as described in the text