Fig. 6

Elevated Expression of Transcripts Protecting the Heart from Oxidative Stress in Flight-immunized Mice. (a) Oxidative stress transcripts were significantly elevated in flight mice after immunization when normalized to flight control mice, according to transcriptomic analysis. (b) The function of several upregulated transcripts related to oxidative stress included protection from cell damage through increased response to stress and ROS. Many of these markers lead to cell and mitochondrial protection. (c) RT-qPCR analysis showed that hepatocyte growth factor (HGF), a cardioprotective gene active during heart damage, was highly elevated in all immunized mice when normalized to their appropriate controls (fc ≥ 4, p-value < 0.05). d-f) RT-qPCR was used to verify that no significant change in common oxidative stress markers was detected in immunized mice when compared to flight control and ground immunized mice (p-value < 0.05)