Fig. 7
From: PbrBGAL6 promotes pollen tube growth by influencing apical pectin level in Pyrus bretschneideri
Inhibited PbrBGAL6 expression affects apical pectin, total ROS, and actin cytoskeleton in pollen tubes. A, C, E, and G Representative images of the total apical pectin, methylated pectin, total ROS, and actin cytoskeleton in control, s-ODN and as-ODN, respectively. B, D, F, and H are the quantification of A, C, E, G, respectively. B and F: Average value of grayscale and fluorescence intensity in a circle within tip 5 μm diameter, respectively. D: Fluorescence density from tip to shank on one side of pollen tube. H: Average depolymerization rate of actin cytoskeleton. Bar = 20 μm; 30 (B), 39 (D), 39 (F), and 102 (H) pollen tubes in each group were analyzed. Extremely significant (P < 0.01) and non-significant differences are indicated by ** and n.s., respectively