Exploration of non-coding RNAs related to intramuscular fat deposition in Xinjiang Brown cattle and Angus × Wagyu cattle

Zhou, Yuxin; Ren, Wanping; Shao, Wei; Gao, Yu; Yao, Kangyu; Yang, Min; Zhang, Xinyu; Wang, Yiran; Li, Fengming; Yang, Liang

doi:10.1186/s12864-025-11453-9

Table 1 The primer of differentially expressed ncRNAs for RT-qPCR

From: Exploration of non-coding RNAs related to intramuscular fat deposition in Xinjiang Brown cattle and Angus × Wagyu cattle

Targeted gene	Primer sequences(5’\(\varvec{\sim }\)3’)
\(bta-miR-142-3p\)	Forward: AGCCAGCGAGTGTTTCCTACTTT
\(bta-miR-142-3p\)	RT Primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCATCCA
\(bta-miR-147\)	Forward: CAACGCCGTGTGCGGAAATG
\(bta-miR-147\)	RT Primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAGCAG
\(bta-miR-217\)	Forward: AGCGAGGCTACTGCATCAGGA
\(bta-miR-217\)	RT Primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATCCAA
\(bta-miR-1306\)	Forward: AAGCTTACCACCTCCCCTGCA
\(bta-miR-1306\)	RT Primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGACGT
\(bta-miR-211\)	Forward: GCCGCGGTTCCCTTTGTCAT
\(bta-miR-211\)	RT Primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGCAAA
\(bta-miR-432\)	Forward: AGCTGGACTCTTGGAGTAGGTCA
\(bta-miR-432\)	RT Primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCACCC
\(TCONS\_00060413\)	Forward: AGTTGTTTTGTGGGGTGGTTTGC
\(TCONS\_00060413\)	Reverse: CTGGTGGCGGTCTCTGGTAATAC
\(TCONS\_00047393\)	Forward: CCAGGCGAAGCAAGACGAGAG
\(TCONS\_00047393\)	Reverse: GCCCAGGCATGTTCCAGGAG
\(TCONS\_00034659\)	Forward: CCACTCGGGAAACCAAATCACTTG
\(TCONS\_00034659\)	Reverse: CTGACTGTGACTGCTATCTCCATCC
\(TCONS\_00047449\)	Forward: GCCATCCAGCCATCTCATCCTC
\(TCONS\_00047449\)	Reverse: GCCACCTCATGCGAAGAATTGAC
\(TCONS\_00024142\)	Forward: GGCTTCCTCGCTGTGGTTCC
\(TCONS\_00024142\)	Reverse: GTCTGCTGCTCACCGTCTCTG
\(TCONS\_00044387\)	Forward: ACACATCAGGAGGCGAGCATTG
\(TCONS\_00044387\)	Reverse: GGCATAGCACCAAGGACCAATTTC
\(novel\_circ\_0007204\)	Forward: GCCCACTTTGTCGACGTTAT
\(novel\_circ\_0007204\)	Reverse: GCTTGGGAGTGAACGTGATT
\(novel\_circ\_0011004\)	Forward: TGACAGAAATGACCCGAACA
\(novel\_circ\_0011004\)	Reverse: TTGCCTTAGAGAGTGCCTCA
\(novel\_circ\_0000394\)	Forward: ATGTTCCTGAAGAGCCCAAA
\(novel\_circ\_0000394\)	Reverse: TCTGGGATCTCCTCTTCTGG
\(novel\_circ\_0010565\)	Forward: CACGTTATGTGGCCCTTTCT
\(novel\_circ\_0010565\)	Reverse: TCAGGTGCCCTTTTGCTAAT
\(novel\_circ\_0008017\)	Forward: AACCCTTACCCCTGAGCAGT
\(novel\_circ\_0008017\)	Reverse: TGGCTGACATCATCATCCAT
\(novel\_circ\_0008526\)	Forward: ATCGGGGAAAACAGAAAAGG
\(novel\_circ\_0008526\)	Reverse: CAAGTGCTTGTTTCAACAGGAG
GAPDH	Forward: TGATGCTCCCATGTTCGTGATG
GAPDH	Reverse: GTGATGGCATGGACAGTGGT
U6	Forward: GCTTCGGCAGCACATATACTAAAAT
U6	Reverse: CGCTTCACGAATTTGCGTGTCAT

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ISSN: 1471-2164

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