Fig. 2

Differences in cytoplasmic maturation of oocytes with different maturation methods. (A) Reactive oxygen species (ROS) levels (green) were detected in oocytes with nuclear maturation from the IVO, COC, and DO groups. BF, bright field. (B) Fluorescence intensity quantification of ROS was measured in oocytes from the IVO, COC, and DO groups after maturation (n = 10–16 oocytes per group). (C) Mitochondrial distribution (green) was detected in oocytes with nuclear maturation from the IVO, COC, and DO groups staining with MitoTracker. BF, bright field. (D) Fluorescence intensity and statistical analysis of mitochondrial signals were performed in oocytes from the IVO, COC, and DO groups after maturation (n = 22–33 oocytes per group). (E) Analysis of abnormal mitochondrial distribution rates in oocytes from the IVO, COC, and DO groups after maturation (n = 22–33 oocytes per group). (F) Distribution of cortical granules (CGs, green) was detected in oocytes with nuclear maturation from the IVO, COC, and DO groups. HOE, Hoechst. (G-H) Analysis of fluorescence intensity quantification and mislocalization rates of CGs were detected in oocytes from the IVO, COC, and DO groups after maturation (n = 15–25 oocytes per group). The data in panels B, D, E, G, and H are shown as the mean ± SD. Statistical significance: n.s., not significant; *, p < 0.05; ***, p < 0.001. Statistical analysis was performed by a two-tailed unpaired Student’s t-test or Fisher’s exact test. Scale bar: 15 μm