Fig. 4

Functional analysis of the rumen microbiome between the three combinations (n = 8). (A) KEGG analysis yielded the top 10 functional pathways with the most significant non-redundant gene enrichment across the three combinations. (B) LEfSe analysis to screen for unique pathways in the three combinations. (C) CAZy analysis of the top 10 carbohydrate enzymes with the most significant differences among the three combinations. (D) LEfSe analysis screening for functional carbohydrases with significant differences among the three combinations