Fig. 3

Heat exposure affects thermogenesis of brown adipocytes. (A) Mito-tracker staining of mitochondria in brown adipocytes after different treatment time (scale bar = 200 μm). (B) The fluorescence intensity was calculated by ImageJ (n = 6); (C) Mitochondria DNA content of brown adipocytes in 37℃ and exposed to 42℃ for 12 h (n = 6); (D) qPCR analysis of thermogenic genes expression of brown adipocytes in 37℃ and exposure to 42℃ for different time (n = 6); (E) Western blotting of thermogenic proteins of brown adipocytes in 37℃ and 42℃, vinculin was considered as loading control; (F) The gray value of UCP1 and PGC1α proteins (n = 2). Data are presented as mean ± SEM. *p < 0.05 and **p < 0.01 versus the corresponding controls are indicated