Fig. 7
From: Short-term heat exposure affects thermogenesis and mitophagy in goat brown adipocytes
Heat exposure promotes mitophagy in goat brown adipocytes. (A) Heat map of TPM values of up-regulated mitophagy-related genes after heat exposure; (B) Western blotting of mitophagy-related proteins brown adipocytes in 37℃ and exposed to 42℃ for 12 h, vinculin was used as loading control (n = 2). (C-F) The gray value of LC3BII/LC3BI, BNIP3, BECN, and PINK1 proteins (n = 2). (G) Transmission electron micrographs of brown adipocytes after heat exposure (scale bar = left, 2 μm; right, 500 nm; M, mitochondrion; ER, endoplasmic reticulum; N, nuclear; Red arrow, auto/mitophagosome). Data are presented as mean ± SEM. *p < 0.05 and **p < 0.01 versus the corresponding controls are indicated