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Table 4 Positive selection sites of high credibility on the PCGs of Triplophysa identified by codeml in PAML and HyPhy, and the amino acid properties changes identified by TreeSAAP

From: Comparative mitogenomic analysis of Chinese cavefish Triplophysa (Cypriniformes: Nemacheilidae): novel gene tandem duplication and evolutionary implications

No.

AA positions

Gene names

Codeml in PAML

 

HyPhy

 

TreeSAAP

 

Site model, PP value (P < 0.05)

Branch-site model, PP value (P < 0.05)

 

FEL, P (P < 0.05)

MEME, P (P < 0.05)

 

Radical changes*

Total number

1

10

ND1

0.999**

 

0.0127

 

pK’

1

2

111

ND1

1.000**

 

 

pK’

1

3

159

ND1

0.974*

 

 

pK’

1

4

161

ND1

0.966*

 

 

pK’

1

5

173

ND1

0.567

 

 

pK’; Ra;

2

6

85

ND2

0.998**

 

 

pK’; αm

2

7

152

ND2

0.994**

 

 

pK’; αc; Rα

3

8

206

ND2

0.94

 

 

Pα; pK’;

2

9

228

ND2

0.903

 

 

Pα; pK’;

2

10

241

ND2

0.982*

 

 

Pα; Pβ; Br;pK’; Ra; Ht

6

11

318

ND2

1.000**

  

 

Pα; pK’; pHi; αm; Rα

5

12

29

ATP6

0.949

 

 

pK’

1

13

195

ATP6

0.999**

 

 

pK’

1

14

87

ND3

0.933

 

 

Pα;pK’; αc;

3

15

91

ND4

0.672

 

 

Pα; c; pK’; αm

4

16

189

ND4

0.644

 

 

pK’; αc;

2

17

194

ND4

0.574

 

 

pK’; αc;

2

18

388

ND4

0.672

 

 

pK’

1

19

2

ND5

0.863

 

0.01

 

c; pK’; h; Rα

4

20

33

ND5

0.980*

 

 

Pα; pHi; αc; αm

4

21

35

ND5

0.936

 

 

Pα; pK’; pHi; αc; αm

5

22

39

ND5

0.991**

 

 

Pα; pK’; pHi; αc; αm

5

23

120

ND5

0.844

 

 

Pα; pK’; αc;

3

24

275

ND5

0.718

 

 

pK’; αc

2

25

277

ND5

0.861

 

 

pK’; αc

2

26

506

ND5

0.519

 

0.04

 

Pα; Br; pK’; αc; Rα

5

27

521

ND5

0.923

 

 

Pα; pK’; Mv; αc; Rα; Hp

6

28

523

ND5

0.794

 

 

Pα; pK’; Rα; Hp

4

29

119

ND6

0.777

 

 

pK’

1

30

306

CYTB

0.986*

 

0.02

 

Pβ; pK’; F; Rα; Ht

5

31

360

CYTB

0.999**

 

0.009

0.00

 

pK’; Hp

2

  1. AA, amino acid; PP, posterior probabilities from Bayes empirical Bayes; *, Physicochemical amino acid properties available in TreeSAAP: αc: Power to be at the C-terminal; αm: Power to be at the middle of alpha-helix; Br: Buriedness; c: Composition; F: Mean r.m.s. fluctuation displacement; h: Hydropathy; Hp: Surrounding hydrophobicity; Ht: Thermodynamic transfer hydrophobicity; Mv: Molecular volume; Mw: Molecular weight; Pα: α- helical tendencies; Pβ: β-structure tendencies; pHi: Isoelectric point; pK’: Equilibrium Constant of ionization for COOH; Ra: Solvent accessible reduction ratio.