Fig. 6
From: The glucose metabolism reprogramming of yak Sertoli cells under hypoxia is regulated by autophagy
Regulation of autophagy level in yak Sertoli cells under hypoxia by autophagy activator rapamycin (RAPA). A: Autophagy activator Rapamycin regulates mRNA levels of autophagy-related genes in yak Sertoli cells under hypoxia. B:Western-blot detection of mTOR, Atg5, Beclin1, LC3I, LC3II, P62 and β-actin proteins. C: Relative expression of autophagy-related proteins. D: Autophagy activator Rapamycin regulates autophagy-related protein levels in yak Sertoli cells under hypoxia. Red fluorescence: Target proteins, respectively. Blue fluorescence: cell nucleus. The scale is 100 μm. E: Fluorescence intensity analysis of proteins