Fig. 7
From: The glucose metabolism reprogramming of yak Sertoli cells under hypoxia is regulated by autophagy
Regulation of LA production and transport by autophagy activator rapamycin (RAPA) in yak Sertoli cells under hypoxia. A: Autophagy activator Rapamycin regulates lactate metabolism related genes in yak Sertoli cells under hypoxia. B: Western-blot detection of GLUT1, GLUT3, GLUT8, MCT1, MCT4 and β-actin proteins. C: Relative expression of lactate metabolism related proteins.D: Regulation of Rapamycin on lactate metabolism related proteins in yak Sertoli cells under hypoxia. Red fluorescence: Target proteins, respectively. Blue fluorescence: cell nucleus. The scale is 100 μm. E:Fluorescence intensity analysis of proteins. F:Regulation of Rapamycin on lactate secretion in yak Sertoli cells under hypoxia. a:The establishment of a standard curve of LA. b: Extracellular and intracellular LA content in yak Sertoli cells. c: Lactate transport rate in yak Sertoli cells. d: The establishment of a standard curve of LDH. e: Detection of LDH in yak sertoli cells