The glucose metabolism reprogramming of yak Sertoli cells under hypoxia is regulated by autophagy

BMC Genomics

Table 1 Primer sequence information

Gene	Primer sequence(5' → 3')	Tm/℃	Fragment size (bp)	Reference sequence
MCT1	F: CTTCCTAATTCTCGGGGGCT	59	151	XM_005887094.1
MCT1	R: ACTTGCCCCCTTTTTCGTCT	59	151	XM_005887094.1
MCT2	F: TTATGTGCGTTGGCTGAGGA	59	169	XM_005898966.1
MCT2	R: CAGGACAGCACTCCACAACT	59	169	XM_005898966.1
MCT4	F: CCATCCTTTGGGGCTGTTTTG	59	101	XM_005905363.2
MCT4	R: GCCTATCCCAAACTCTCGCA	59	101	XM_005905363.2
GLUT1	F: CGGCATCAACGCTGTTTTCT	59	106	XM_005901364.2
GLUT1	R: GAAGGCTGTGTTGACGATGC	59	106	XM_005901364.2
GLUT3	F: CAAAGTTCCTGAGACCCGTG	59	141	XM_005912004.2
GLUT3	R: TAGGCATTGGTGTCCTTCGT	59	141	XM_005912004.2
GLUT8	F: CGTCAATGCCGTCATGTTCT	59	173	XM_005901861.2
GLUT8	R: TGACCACACCTGACAAGGTC	59	173	XM_005901861.2
Atg5	F: AGTTGCTCCTGAAGATGGGG	60	147	NM_001034579.2
Atg5	R: TCTGTTGGTTGCGGGATGAT	60	147	NM_001034579.2
ATG12	F: CAGAGGACCAACCTGCGATT	59	153	XM_027552640.1
ATG12	R: TCAGCCATCTTGCTTGGAGG	59	153	XM_027552640.1
Beclin1	F: GAAACCAGGAGAGACCCAGG	56	114	NM_001033627.2
Beclin1	R: GTGGACATCATCCTGGCTGG	56	114	NM_001033627.2
LC3	F: CCGACTTATCCGAGAGCAGC	59	161	NM_001001169.1
LC3	R: TGAGCTGTAAGCGCCTTCTT	59	161	NM_001001169.1
Bax	F: ACAGGGGCCCTTTTGCTTCA	60	206	NM_173894.1
Bax	R: TCTCGGGGAGAGTCTGTGTC	60	206	NM_173894.1
Bcl-2	F: GAGGGGTCATGTGTGTGGAG	59	207	NM_001166486.1
Bcl-2	R: GACTGAGCAGTGCCTTCAGA	59	207	NM_001166486.1
Caspase3	F: AGCGTCGTAGCTGAACGTAAA	59	247	NM_001077840.1
Caspase3	R: CTGCATCCACGTCTGTACCA	59	247	NM_001077840.1
β-actin	F: GAGCTACGAGCTTCCTGACG	60	121	NM_173979.3
β-actin	R: GGTAGTTTCGTGAATGCCGC	60	121	NM_173979.3

ISSN: 1471-2164