Fig. 2
From: scTCR-seq and HTS reveal a special novel TRBD2-TRBJ1 rearrangement in mammalian TRB CDR3 repertoire
Heatmap of D-J rearrangement frequencies of 42 samples and statistical analysis of the TCR V-D-J rearrangement frequencies of bats, rhesus monkeys, and bovines. (A) HTS data of 4 human blood samples. (B) scTCR-seq data of 5 human blood samples. (C) HTS data of 4 human thymus samples. (D) scTCR-seq data of 5 human thymus samples. (E) HTS data of 3 mouse spleen samples. (F) scTCR-seq data of 9 mouse peripheral tissue samples. (G) HTS data of 3 mouse thymus samples. (H) HTS data of 2 bat spleen samples. (I) HTS data of 1 rhesus monkey blood sample. (J) scTCR-seq data of 5 rhesus monkey peripheral tissue samples; (K) HTS data of 1 bovine spleen sample. (L) Frequency of D genes and rearranged D-J usage in bat spleen samples (SRR21464508); (M) Frequency of D genes and rearranged D-J usage in bat spleen samples (SRR21464509); (N) rhesus monkey blood sample; (O) rhesus monkey peripheral tissue samples; (P) bovine spleen sample; (Q) Examples of raw and annotated sequences from rhesus monkey scTCR-seq data (SRR15249798). Note: (1) D1 is the complete sequence (complete Vx-D1-Jx) of all D1 genes involved in the rearrangement of each sample; D2 is the complete sequence (complete Vx-D2-Jx) of all D2 genes involved in the rearrangement of each sample; D3 is the complete sequence (complete Vx-D3-Jx) of all D3 genes involved in the rearrangement of each sample; D1-J1 denotes total Vx-D1-J1; D1-J2 denotes total Vx-D1-J2; D1-J3 denotes total Vx-D1-J3; D2-J1 denotes total Vx-D2-J1; D2-J2 denotes total Vx-D2-J2; D2-J3 denotes total Vx-D2-J3; D3-J1 denotes total Vx-D3-J1; D3-J2 denotes total Vx-D3-J2; D3-J3 denotes total Vx-D3-J3; Bat and rhesus monkey: D1 + D2 = 100%; D1-J1 + D1-J2 = 100%; D2-J1 + D2-J2 = 100%; V30(or V31)-D2-J1 + Vx-D2-J1 = 100%; Bovine: D1 + D2 + D3 = 100%; D1-J1 + D1-J2 + D1-J3 = 100%; D2-J1 + D2-J2 + D2-J3 = 100%; D3-J1 + D3-J2 + D3-J3 = 100%; V30-D3-J1 + Vx-D3-J1 = 100%; V30-D2-J1 + Vx-D2-J1 = 100%; V30-D2-J3 + Vx-D2-J3 = 100%. (2) A bar chart was used for sample groups with n = 1, and statistical analysis was performed using Pearson’s chi-squared test (L-N, P). A dot plot was used for sample groups with n ≥ 3, and statistical analysis was performed using the Mann-Whitney U test (O). All statistically significant differences are indicated as *P value < 0.05 and **P value < 0.01