Fig. 3

TRBD2-TRBJ1 rearrangement mechanism diagram; example diagram of human and mouse D2-J1 rearrangement sequences, comparison statistics of V30(31)-D2-J1 frequency and average frequency of each V gene subfamily in forward Vx-D2-J1. (A) The reverse V30 first recombines with the D2 gene, resulting in inversional rearrangement of V30-D2 with the J1 family to form V30-D2-J1. (B) The reverse V30 first recombines with the D1 gene in inversional rearrangement, contributing to inversion of Vx-D2 and the Vx-D2-J1(x) rearrangement mechanism. (C) Examples of reverse V30-D2-J1 arrangement sequences in human single T cell. (D) Examples of forward Vx-D2-J1 arrangement sequences in human single T cell. (E) Examples of reverse V31-D2-J1 arrangement sequences in mouse single T cell. (F) Examples of forward Vx-D2-J1 arrangement sequences in mouse single T cell. (G) Examples of concurrent V30-D1-Jx and Vx-D2-J1(x) rearrangement sequences in a single T cell of human. (H) Examples of concurrent V31-D1-Jx and Vx-D2-J1(x) rearrangement sequences in a single T cell of mouse. (I) Statistical analysis of the frequency of V30(31)-D2-J1 in human and mouse total samples compared with the average frequency of forward Vx-D2-J1. Note. We used a dot plot for sample groups with n ≥ 3 and performed statistical analysis using the Mann-Whitney U test. All statistically significant differences are indicated as *P value < 0.05 and **P value < 0.01