Fig. 3

Estimates of the proportion of neurons in observed bulk RNA-seq DLPFC samples using NNLS. Analysis of N = 8 observed bulk RNA-seq DLPFC samples from neurotypical donors from [27]. A Scatterplots of (x-axis) known versus (y-axis) predicted (blue points, bottom row) neuron and (red points, top row) glial cell proportions using NNLS without scaling (left column) or with cell size factor scaling (right column) for bulk RNA-seq samples from DLPFC in which known cell type proportions are estimated from snRNA-seq data. Text label indicates Br8667_mid, an outlier sample. Diagonal lines indicate y = x and no error. B Jittered points and quantile boxplots showing (y-axis) error, calculated as the difference between true minus predicted cell type proportions, either (left) with or (right) without scaling, with point and box line colors indicating either (red) glial or (blue) neuron cell type