Fig. 7
Analysis of the cis-regulatory elements of the promoter. a Schematic representation of the reporter plasmids used in the dual LUC assay (retaining the gibberellin-responsive originals Q0, Q1 and Q2). b Relative activity levels of LUC (Q0, Q1 and Q2). Each bar indicates the mean ± SD from three biological replicates (∗ ∗ ∗ P < 0.001). c Analysis of the Dual Luciferase Assay System (Q0, Q1, Q2, CK: sterile water application treatment as the control group; GA: 100 mg/L GA solution-coated treatment as the experimental group.) d Schematic representation of the reporter plasmids used in the dual LUC assay (removing the gibberellin-responsive originals Q1*, Q2*). e Relative activity levels of LUC (Q1*, Q2*). Each bar indicates the mean ± SD from three biological replicates (∗ ∗ ∗ P < 0.001). f Analysis of the Dual Luciferase Assay System (Q1*, Q2*, CK: sterile water application treatment as the control group; GA: 100 mg/L GA solution-coated treatment as the experimental group.) Note: Q0 is the promoter fragment −1,150 bp to −125 bp, Q1 is the promoter fragment −1150 bp to −584 bp, and Q2 is the promoter fragment −604 bp to −125 bp