Fig. 5

Expression patterns of bHLH genes in different organs based on transcriptome datasets. (A) Expression heatmap of AspbHLHs in xylem (Xy), phloem (Ph), sclerenchymatic belt (Sb) in the vascular bundle, pith (Pi) and leaf (Le). The center of the heat map is a cross-section of the A. spinulosa stem, with red arrows marking the locations of the different tissues. Genes that were not expressed in any tissue were excluded from the figures. The color bars represent the relative expression levels after column scale, where red indicates high expression levels and blue represents low expression levels. The data in the heatmap were the original FPKM values of the samples. (B-P) Analysis of AspbHLHs transcript levels in xylem (Xy), phloem (Ph), sclerenchymatic belt (Sb) in the vascular bundle, pith (Pi) and leaf (Le). Blue line represents the transcript levels of AspbHLHs determined by RT-qPCR, and red bar graph indicates the FPKM value. Values are from three technical replicates and three biological replicates, with error bars indicating standard deviations