Fig. 6

Results of WGCNA analysis and KEGG, GO analysis of yellow module. (A) Hierarchical clustering of gene modules in 10 tissues of A. spinulosa, including leaves, stems at three different developmental periods, xylem, phloem, pith, and sclerenchymatic belt. (B) Heatmap showing the gene co-expression network across different modules in different tissues. (C) GO analysis for the yellow module. (D) KEGG analysis for the yellow module. (E) Co-expression network of three differentially expressed bHLH genes (AspbHLH80, AspbHLH120, and AspbHLH185) in xylem, co-expressed with genes related to lignin biosynthesis. Different colors represent the number of co-expressed genes, with redder colors indicating a higher number of co-expressed genes. Genes highlighted in red indicate co-expression with the three bHLH genes. (F) Expression patterns of lignin biosynthesis-related genes co-expressed with AspbHLH80, AspbHLH120, and AspbHLH185 in various tissues. The heatmap data represents the original FPKM values of the samples