Fig. 1

Illustrates the experimental design and the general distribution patterns of m6A peaks under different stress conditions in A. japonicus. (A) Represents the experimental treatment groups, detailing the environmental stress conditions applied, including variations in temperature and dissolved oxygen levels. (B) Summarizes the total number of m6A peaks identified in each group, reflecting the overall methylation landscape under each condition. (C) Shows a Venn diagram displaying shared and unique m6A peaks among the different treatment groups, while (D) Categorizes the proportion of genes containing different numbers of m6A peaks. (E) Visualizes the distribution of m6A peaks across transcript regions, including 3’UTR, 5’UTR, CDS, start codon, and stop codon, emphasizing functional localization patterns. (F) Depicts the density distribution of m6A peaks along transcripts, highlighting positional preferences and regions with higher methylation density. (G) Identifies consensus motifs associated with m6A peaks, revealing sequence-specific regulatory features linked to stress adaptation. (H) and (I) present Venn diagrams comparing shared and unique m6A peaks and m6A-associated genes across group pairs, respectively. Finally, (J) displays the volcano plot illustrating the differential m6A peaks between groups and their associated genes