Fig. 2

Abundances of miRNAs by length, family, and number of isoforms. Length distribution of miRNAs identified from the (A) leaves and (B) berries from GRBV-positive and negative vines collected during pre- and post-véraison. Reads were normalized by RPM. In leaf samples, 23nt reads were the most abundant during pre-véraison, but 21nt reads were the most abundant during post-véraison. In berries, 21nt reads were the most abundant in samples collected during pre- and post-véraison. Family-wise distribution of miRNAs in (C) leaves and (D) berries from GRBV-negative and GRBV-positive samples collected during pre- and post-véraison. Reads were normalized by RPM. E Number of distinct miRNA isoforms detected from miRNA families. Reads from the miR3634 family were the most abundant in every sampling category except for berry samples at post-véraison from GRBV-positive vines, where miR3623 reads were more abundant. The overabundance of miR3634 family reads was due to the isoform miRNA3634a-3p. This overabundance also contributed to the high 23nt read abundance in leaves. Sampling categories are denoted according to the following abbreviations: pre-véraison [P], post-véraison [PO], leaves [L], berries [B], GRBV-negative [H], GRBV-positive [D]